Polyethylenimine，Linear，Mw 25，000

線性化聚乙烯亞胺（PEI 25，000）

基本描述：

線性化聚乙烯亞胺PEI 25，000，一種高電荷陽離子聚合物，非常容易結(jié)合于高電荷陰離子基質(zhì)。工業(yè)應用上，線性化PEI可改善負電荷染料的物理外觀，以調(diào)整染料的化學特性和增強染料與固相基質(zhì)的黏附能力，常用作黏附增強劑，作用同多聚賴氨酸（poly-lysine）；科研應用上，線性化PEI能與DNA或其他負電荷生物大分子簡單結(jié)合，正是這一特性，使得成為一種非常行之有效的載體運輸介質(zhì)（Vector carrier），即轉(zhuǎn)染試劑。

化學性質(zhì)：

訂購信息：【*，現(xiàn)貨供應】

使用方法【來源于：CSH protocols，僅作交流學習】 ：

1. Pour ~450 mL of Milli-Q H₂O into a 500-mL glass beaker.

2. Add 500 mg of PEI to the beaker with stirring.

3. Add concentrated HCl drop-wise to the solution to pH<2.0.

4. Stir until the PEI is dissolved (~2-3 h). Maintain the pH<2.0 throughout.

Approximay 800 μL of 12 M HCl will be required for full PEI dissolution. There may still be some small fiber particles that will not dissolve.

5. Add concentrated NaOH drop-wise to the solution to pH 7.0.

Approximay 500 μL of 10 M NaOH will be required to neutralize the PEI solution.

6. Pour the solution into a 500-mL glass cylinder. Adjust the final volume to 500 mL with Milli-Q H₂O.

7. Filter-sterilize the solution through a 0.22-μm membrane.

8. Store aliquots of the desired volume at -20°C.

應用文獻【基因轉(zhuǎn)染用途】 ：

Zhong Z, Feijen J, Lok MC, Hennink WE, Christensen LV, Yockman JW, Kim YH, Kim SW. (2005). Low molecular weight linear polyethylenimine-b-poly(ethylene glycol)-b-polyethylenimine triblock copolymers: synthesis, characterization, and in vitro gene transfer properties.Biomacromolecules. 6(6):3440-8.

Sharon E. Reed, Elizabeth M. Staley, John P. Mayginnes, David J. Pin (2006). Transfection of mammalian cells using linear polyethylenimine is a simple and effective means of producing recombinant adeno-associated virus vectors. Journal of Virological Methods. 138(1–2): 85–98.

Patti A. Longo, Jennifer M. Kavran, Min-Sung Kim, and Daniel J. Leahy（2013）Transient Mammalian Cell Transfection with Polyethylenimine (PEI) . Methods Enzymol. 529: 227–240.

——Written/Edited by V. Shallan【版權歸MKBio懋康所有】  

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線性化聚乙烯亞胺PEI 25000細胞傳代

線性化聚乙烯亞胺PEI 25000細胞傳代