西班牙Certest大腸桿菌O157抗體（克隆E10）

廣州健侖生物科技有限公司

廣州健侖長期供應(yīng)各種生物原料，主要代理品牌：西班牙Certest。

主要產(chǎn)品包括各種生物單克隆抗原抗體、重組蛋白。

Certest公司 大腸桿菌O157抗體（克隆E10） Certest品牌

我司還提供其它進口或國產(chǎn)試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團菌、化妝品檢測、食品安全檢測等試劑盒以及日本生研細菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產(chǎn)品。

歡迎咨詢

歡迎咨詢2042552662

【產(chǎn)品介紹】

Certest公司 大腸桿菌O157抗體（克隆E10） Certest品牌

傳播特點核酸檢測試劑盒
宿主范圍廣對人致病性低
腺病毒載體系統(tǒng)可廣泛用于人類及非人類蛋白的表達。腺病毒可感染一系列哺乳動物細胞，因此在大多哺乳動物細胞和組織中均可用來表達重組蛋白。特別需要指出的是：腺病毒具有嗜上皮細胞性，而人類的大多數(shù)的腫瘤就是上皮細胞來源的。另外，腺病毒的復(fù)制基因和致病基因均已相當清楚，在人群中早已流行（70-80%成人體內(nèi)都有腺病毒的中和抗體存在）。人類感染野生型腺病毒后僅產(chǎn)生輕微的自限性癥狀，且病毒唑治療有效。
在增殖和非增殖細胞中感染和表達基因
逆轉(zhuǎn)錄病毒只能感染增殖性細胞，因此DNA轉(zhuǎn)染不能在非增殖細胞中進行，而必須使細胞處于持續(xù)培養(yǎng)狀態(tài)。腺病毒則能感染幾乎所有的細胞類型，除了一些抗腺病毒感染的淋巴瘤細胞。腺病毒是研究原代非增殖細胞基因表達的PCR檢測試劑盒系統(tǒng)，它可以使轉(zhuǎn)化細胞和原代細胞中得到的結(jié)果直接進行對比。
能有效進行增殖 滴度高
腺病毒系統(tǒng)可產(chǎn)生1010到1011VP/ml，濃縮后可達1013VP/ml，這一特點使它非常適用于基因治療。
與人類基因同源
腺病毒載體系統(tǒng)一般應(yīng)用人類病毒作為載體，以人類細胞作為宿主，因此為人類蛋白進行準確的翻譯后加工和適當?shù)恼郫B提供了一個理想的環(huán)境。大多數(shù)人類蛋白都可達到高水平表達并且具有*的功能。
已知腺病毒有41個血清型別，其中很多與人類上、下呼吸道感染密切有關(guān)。3型和7型腺病毒為腺病毒肺炎的主要病原。
腺病毒是DNA病毒，主要在細胞核內(nèi)繁殖，耐溫、耐酸、耐脂溶劑的能力較強，除了咽、結(jié)合膜及淋巴組織外，還在腸道繁殖?？筛鶕?jù)其對特殊動物紅細胞的凝集能力分為3組，容易引起嬰幼兒肺炎的3、7、11、14、21這一組，均能凝集猴紅細胞。
臨床表現(xiàn)
1.起病
潛伏期3～8天。一般急驟發(fā)熱，往往自第1～2日起即發(fā)生39℃以上的高熱，至第3～4日多呈稽留或不規(guī)則的高熱；3/5以上的病例zui高體溫超過40℃。
2.呼吸系統(tǒng)癥狀
大多數(shù)病兒自起病時即有咳嗽，往往表現(xiàn)為頻咳或輕度陣咳，同時可見咽部充血。呼吸困難及發(fā)紺多數(shù)開始于第3～6日；重癥病例出現(xiàn)鼻翼扇動、三凹征、喘憋及口唇指甲青紫。重癥病兒可有胸膜反應(yīng)或胸腔積液。
3.神經(jīng)系統(tǒng)癥狀
一般于發(fā)病3～4天以后出現(xiàn)嗜睡、萎靡等，有時煩躁與萎靡相交替。在嚴重病例中晚期出現(xiàn)半昏迷及驚厥。部分病兒頭向后仰，頸部強直。除中毒性腦病外，尚有一部腺病毒所致的腦炎，故有時需作腰穿鑒別。
4.循環(huán)系統(tǒng)癥狀
面色蒼白較為常見，重者面色發(fā)灰。心率增快，輕癥一般不超過每分鐘160次，重癥多在160～180次/分鐘，有時達200次/分鐘以上。重癥病例發(fā)病第6～14日出現(xiàn)心力衰竭。肝臟逐漸腫大，少數(shù)也有脾腫大。
5.消化系統(tǒng)癥狀
半數(shù)以上有輕度腹瀉、嘔吐，嚴重者常有腹脹。腹瀉可能與腺病毒在腸道內(nèi)繁殖有關(guān)，但在一部分病例也可能由于病情重、高熱而影響了消化功能。
6.其他癥狀
可有卡他性結(jié)膜炎、紅色丘疹、斑丘疹、猩紅熱樣皮疹。

Certest公司 

想了解更多的產(chǎn)品及服務(wù)請掃描下方二維碼：

【公司名稱】 廣州健侖生物科技有限公司
【市場部】    楊永漢

【】 
【騰訊  】 2042552662
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號二期2幢101-103室

Propagation characteristics of nucleic acid detection kit
Host wide range of low pathogenicity to humans
Adenovirus vector systems are widely used for the expression of human and non-human proteins. Adenovirus can infect a series of mammalian cells and therefore can be used to express recombinant proteins in most mammalian cells and tissues. Of particular note is that adenoviruses have psoriatic properties, whereas most human tumors are of epithelial origin. In addition, the replication and causative genes of adenoviruses are well known and have been prevalent in the human population (70-80% of adults have an adenovirus neutralizing antibody present). Human infection with wild-type adenovirus produces only mild self-limiting symptoms, and ribavirin is effective.
Infected and expressed genes in proliferating and non-proliferating cells
Retroviruses can only infect proliferating cells, so DNA transfection can not be performed in non-proliferating cells, and the cells must be kept in a continuous culture. Adenoviruses can infect almost all cell types, except for some adenovirus-infected lymphoma cells. The adenovirus is a PCR assay kit that studies the expression of primary nonproliferative cells in gene expression, allowing direct comparison of the results obtained in transformed and primary cells.
Can effectively proliferate high titer
The adenovirus system produces 1010 to 1011 VP / ml and up to 1013 VP / ml after concentration, which makes it well suited for gene therapy.
Homologous with human genes
Adenovirus vector systems typically use human viruses as vectors and human cells as hosts, thus providing an ideal environment for accurate translational processing and proper folding of human proteins. Most human proteins achieve high levels of expression and are fully functional.
Adenovirus is known to have 41 serotypes, many of which are closely linked to upper and lower respiratory tract infections in humans. Adenovirus type 3 and type 7 adenovirus pneumonia is the main pathogen.
Adenoviruses are DNA viruses, which are mainly propagated in the nucleus of cells. They are capable of resisting temperature, acid and grease, and are capable of propagating in the gut besides the pharynx, the conjunctiva and lymphoid tissues. According to its ability to agglutinate red blood cells of special animals is divided into 3 groups, easily lead to pneumonia in infants and young children 3,7,11,14,21 this group, can agglutinate monkey red blood cells.
Clinical manifestations
1. onset
Latency 3 to 8 days. The general rapid fever, often from the first day on the 2nd occurred above 39 ℃ high fever, to the 3rd to 4th, mostly absent or irregular high fever; more than 3/5 cases of the highest body temperature over 40 ℃.
2. Respiratory symptoms
Most sick children since the onset of cough, often manifested as frequent cough or mild cough, while visible throat congestion. Dyspnea and cyanosis most started on the 3rd to 6th; severe cases of nasal flap fan, three concave sign, wheezing and lip nails bruising. Severe illness may have pleural reaction or pleural effusion.
3. Neurological symptoms
Usually 3 to 4 days after the onset of lethargy, apathetic, etc., and sometimes irritability and malaise alternate. Severe cases of late appear semi-coma and convulsions. Some sick children head backwards, neck stiffness. In addition to toxic encephalopathy, there is an adenovirus caused by encephalitis, it is sometimes necessary to wear for identification.
4. circulatory system symptoms
Pale pale is more common, heavy gray hair. Increased heart rate, mild illness generally does not exceed 160 times per minute, severe in 160 to 180 times / minute, and sometimes up to 200 times / minute. Severe cases of onset of heart failure on the 6th to 14th. Gradually enlarged liver, a few also have splenomegaly.
5. Digestive system symptoms
More than half of mild diarrhea, vomiting, often severe abdominal distension. Diarrhea may be associated with adenovirus in the gut reproductive, but in some cases may also be due to illness, high fever and affect the digestive function.
6. other symptoms
There may be catarrhal conjunctivitis, red papules, rash, scarlet fever-like rash.