人腎癌細(xì)胞；A498價(jià)格，人腎癌細(xì)胞；A498用途，說(shuō)明書(shū)，可直接向我們銷(xiāo)售人員索取。
細(xì)胞中文名稱(chēng)：
細(xì)胞英文名稱(chēng)：Human renal cell carcinoma;?A498
細(xì)胞產(chǎn)品編號(hào)：FS01-00229
生長(zhǎng)特性： 貼壁生長(zhǎng)
培養(yǎng)條件 DMEM-H: Dulbecco's Modified Eagle's Medium (DME H-21 4.5g/Liter Glucose) 10%FBS
傳代方法 1:2~1:10傳代；每周換液2次。
傳代情況 P180
凍存條件 基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS
操作臺(tái)的滅菌處理：
1)無(wú)菌室及無(wú)菌操作臺(tái)(laminarflow)用紫外燈照射30-60分鐘滅菌，70%ethanol擦拭無(wú)菌操作抬面，并開(kāi)啟無(wú)菌操作臺(tái)風(fēng)扇運(yùn)轉(zhuǎn)10分鐘。
2)細(xì)胞用的培養(yǎng)基如有相同切記不可共享培養(yǎng)基，以避免失誤混淆或細(xì)胞間污染。 (注：實(shí)驗(yàn)操作應(yīng)在抬面之*無(wú)菌區(qū)域，勿在邊緣之非無(wú)菌區(qū)域操作。)
培養(yǎng)細(xì)胞將復(fù)合細(xì)胞計(jì)數(shù)要求的細(xì)胞懸液分裝入培養(yǎng)瓶?jī)?nèi)，將培養(yǎng)瓶放入37℃和5%CO2的培養(yǎng)箱內(nèi)2-4小時(shí)(或者24-48小時(shí))后換液繼續(xù)培養(yǎng)培養(yǎng)，換液的時(shí)間由細(xì)胞情況而定。
蘇因嚴(yán)格遵守“快速融化”原則：必須將凍存在-196℃液氮中的細(xì)胞快速融化至37℃，使細(xì)胞外凍存時(shí)的冰晶迅速融化，避免冰晶緩慢融化時(shí)進(jìn)入細(xì)胞形成再結(jié)晶，對(duì)細(xì)胞造成損害。
12α-Hydroxyevodol 12Alpha-羥基吳茱萸醇 C26H28O10 ≥96% 光滑黃皮Clausena lenis 120722-04-5
12α-Hydroxygrandiflorenic acid none C20H28O3 ≥95% 南美蟛蜞菊Wedelia trilobata 63768-17-2
13-Hydroxy-8,11,13-podocarpatrien-18-oic acid 13-羥基-8,11,13-羅漢松科三烯-18-酸 C17H22O3 ≥95% 云南松Pinus yunnanensis 61597-83-9
15-Hydroxy-7-oxodehydroabietic acid 15-羥基-7-氧代去氫松香酸 C20H26O4 ≥95% 云南松Pinus yunnanensis 95416-25-4
15-Hydroxydehydroabietic acid 15-羥基脫氫樅酸 C20H28O3 ≥97% 麻楝Chukrasia tabularis 54113-95-0
16-Hydroxy-2-oxocleroda-3,13-dien-15,16-olide 5-羥基-4-[2-(1,2,3,4,4A,7,8,8A-八氫-1,2,4A,5-四甲基-7-氧代-1-萘基)乙基]-2(5H)-呋喃酮 C20H28O4 ≥98% 景洪暗羅'Polyalthia cheliensis 165459-53-0
16-Hydroxy-8(17),13- labdadien-15,16-olid-19-oic acid 16-羥基-8(17),13-賴(lài)百當(dāng)二烯-15,16-內(nèi)酯-19-酸 C20H28O5 ≥95% 側(cè)柏Platycladus orientalis 691009-85-5
19(S)-Hydroxyconopharyngine none C23H30N2O5 ≥95% 非洲馬鈴果Voacanga africana 16790-93-5
19-Hydroxybaccatin III 19-羥基漿果赤霉素 III C31H38O12 ≥95% 云南紅豆杉Taxus yunnanensis 78432-78-7
1-Hydroxy-2,3,4,7-tetramethoxyxanthone 1-羥基-2,3,4,7-四甲氧基山山酮 C17H16O7 ≥95% 橢圓葉花錨Halenia elliptica 14103-09-4
1-Hydroxy-2-oxopomolic acid 1-羥基-2-氧代果樹(shù)酸 C30H46O6 ≥95% 龍牙草Agrimonia pilosa 217466-37-0
1-Hydroxy-2-prenylnaphthalene 2-異戊烯基-1-萘酚 C15H16O ≥95% 梓Catalpa ovata 16274-34-3
1-Hydroxybaccatin I none C32H44O14 ≥98% 云南紅豆杉Taxus yunnanensis 30244-37-2
1-Hydroxycanthin-6-one 1-羥基-6-鐵屎米酮 C14H8N2O2 ≥95% 臭椿Ailanthus altissima 80787-59-3
1-Hydroxyrutaecarpine 1-羥基吳茱萸次堿 C18H13N3O2 ≥98% 吳茱萸Evodia rutaecarpa 53600-24-1
2-(2'-Hydroxytetracosanoylamino)- octadecane-1,3,4-triol (2S,3S,4R,2'R)-2-(2'-羥基二十四碳酰氨基)十八烷-1,3,4-三醇 C42H85NO5 ≥96% 吳茱萸Evodia rutaecarpa 154801-30-6
2-(2'-Hydroxytetracosanoylamino)- octadecane-1,3,4-triol tetraacetate (2R)-2-(乙酰氧基)-N-[(1S,2S,3R)-2,3-雙(乙酰氧基)-1-[(乙酰氧基)甲基]十七烷基]二十四碳酰胺 C50H93NO9 ≥96% 余甘子Phyllanthus emblica 340702-68-3
2-(4-Chloro-3-hydroxy-1-butynyl) -5-(1,3-pentadiynyl)thiophene 1-氯-4-(5-(1,3-戊二炔)噻吩-2-基)-3-丁炔-2-醇 C13H9ClOS ≥98.5% 臭靈丹Laggera pterodonta 26905-70-4
2-(4-Hydroxy-3-methoxyphenyl) -7-methoxy-5-benzofuranpropanol 2-(4-羥基-3-甲氧基苯基)-7-甲氧基-5-苯并呋喃丙醇 C19H20O5 ≥98.5% 君遷子Diospyros lotus 144735-57-9
2-(4-Hydroxyphenyl)-6-methyl- 2,3-dihydro-4H-pyran-4-one 2,3-二氫-2-(4-羥基苯基)-6-甲基-4H-吡喃-4-酮 C12H12O3 ≥98.5% 半枝蓮Scularia barbata 1167483-18-2
2-(4-Hydroxyphenyl)ethanol 對(duì)羥基苯乙醇 C8H10O2 ≥96% 滇西八角 Illicium merrillianum 501-94-0
22(S),23(S)- Homobrassinolide 蕓苔素內(nèi)酯 C29H50O6 ≥98% 油菜花粉Rape flower 80483-89-2
22-Hydroxy-3-oxo-12-ursen-30-oic acid (22ALPHA)-22-羥基-3-氧代烏蘇-12-烯-30-酸 C30H46O4 ≥95% 衛(wèi)矛Euonymus alatus 173991-81-6
23-Hydroxybetulin none C30H50O3 ≥95% 紅芽木Cratoxylum formosum 84414-40-4
23-Hydroxymangiferonic acid 23-羥基杧果酮酸 C30H46O4 ≥95% 杧果Mangifera indica 232266-08-9
25-Hydroxycycloart-23-en-3-one 25-羥基環(huán)木菠蘿-23-烯-3-酮 C30H48O2 ≥95% 波羅蜜Artocarpus heterophyllus 148044-47-7
27-Hydroxymangiferolic acid 27-羥基芒果醇酸 C30H48O4 ≥95% 杧果Mangifera indica 17983-82-3
27-Hydroxymangiferonic acid 27-羥基杧果酮酸 C30H46O4 ≥95% 杧果Mangifera indica 5132-66-1
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