尼古丁唾液檢測試劑盒

廣州健侖生物科技?有限公司

本司長期供應(yīng)尼古丁（可替寧）檢測試劑盒，其主要品牌包括美國NovaBios、廣州健侖、廣州創(chuàng)侖等進(jìn)口產(chǎn)品，國產(chǎn)產(chǎn)品，試劑盒的實(shí)驗(yàn)方法是膠體金方法。

我司還提供其它進(jìn)口或國產(chǎn)試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團(tuán)菌等試劑盒以及日本生研細(xì)菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產(chǎn)品。

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【包裝規(guī)格】

1人份/袋，40人份/盒

【預(yù)期用途】

尼古丁(Nicotine)是煙草中的主要生物堿，是導(dǎo)致吸煙成癮的物質(zhì)動因，也是評價人體攝入煙草煙霧的常用指標(biāo)。但因?yàn)槟峁哦“胨テ诙?，無法作為標(biāo)志物檢測，其代謝物可替寧因?yàn)榘胨テ陂L作為吸煙和戒煙的標(biāo)志物。

本品采用競爭抑制法和膠體金免疫層析技術(shù)，用于快速定性檢測人體唾液中的可替寧，適用于評價煙草煙霧攝入的初步篩查。

【主要組成成份】

• 可替寧檢測試劑盒（40人份）：每人份鋁箔袋單獨(dú)包裝。其中試劑盒由金標(biāo)可替寧單克隆抗體、可替寧-BSA結(jié)合物、羊抗兔IgG多克隆抗體、硝酸纖維素膜、聚酯纖維素膜、塑料背襯、塑料模板組成。
• 一次性滅菌唾液取樣棒（40人份）
• 唾液收集器（40人份）
• 使用說明書（1份）

• 【檢驗(yàn)方法】

• 撕開鋁箔袋，取出試劑，應(yīng)在1小時內(nèi)盡快使用。
• 拿出一次性唾液收集器。（步驟1）
• 手持收集器蓋帽使海綿頭含入口中，直至海綿頭吸滿液體（膨脹變軟）。（步驟2）
• 將海綿頭放回收集器并擰緊蓋帽，使所含樣本流入收集器下端。（步驟3）
• 將試劑盒置于干凈平坦的臺面上，同時打開滴樣孔蓋帽。（步驟4）
• 擠壓收集器上端管壁，垂直滴加2滴無空氣泡的唾液（約100ul）于加樣孔（S）中。(步驟5)
• 等待紫紅色條帶的出現(xiàn)，3-5分鐘時直接觀察結(jié)果，10分鐘后判定無效。 

尼古丁唾液檢測試劑盒

Mann等以分子量很小的乙醇胺（Mr= 61.08）為靶物質(zhì)，將其固定在磁珠上。加入核酸序列文庫后振蕩孵育30 min，利用磁性格材料的分離富集效應(yīng)去除未與乙醇胺結(jié)合的核酸序列，而后加入含有尿素及EDTA的緩沖液提取結(jié)合在靶物質(zhì)上的核酸序列，進(jìn)入下一步擴(kuò)增富集，zui終篩選出6條性格能良好的適配體。5、柱層析分離法將靶物質(zhì)結(jié)合在樹脂層析柱上，適配體序列文庫與靶物質(zhì)孵育后，能特異性格結(jié)合的序列可以留在層析柱上，達(dá)到分離的目的。有人將靶物質(zhì)伏馬毒素B1固定在凝膠樹脂親和層析柱上，注入含有核酸序列文庫的溶液，用結(jié)合緩沖液反復(fù)沖洗層析柱，成功分離出與伏馬毒素B1結(jié)合解離常數(shù)為100±30 nmol/L的適配體序列。其中，前3種方法常用于大分子靶標(biāo)物質(zhì)的分離，后2種方法常用于小分子靶標(biāo)物質(zhì)的分離。
隨機(jī)核酸庫通常由化學(xué)合成獲得，也可以通過基因組DNA設(shè)計(jì)以及體外轉(zhuǎn)錄等途徑獲得。篩選適配體時需設(shè)計(jì)合理的核酸庫。常規(guī)的隨機(jī)核酸庫設(shè)計(jì)為，序列兩端含引物序列，中間一般由20-60個堿基組成。如增加隨機(jī)序列的長度，將使隨機(jī)庫中核酸分子的結(jié)構(gòu)多樣性格呈指數(shù)級（4n，n為隨機(jī)序列中的堿基個數(shù)）增長。減少引物序列，避免引物序列對適配體二級結(jié)構(gòu)形成的干擾，減少引物區(qū)域與靶分子的非特異性格結(jié)合；通過高通量測序分析次級庫的序列特征，改變核酸庫的合成方案，可以增加與靶分子結(jié)合的序列或基序。
一、引物序列的堿基數(shù)設(shè)計(jì)目前用于篩選的常規(guī)核酸庫的核酸序列全長為60-100nt，引物序列為30-32nt，引物序列約占核酸序列長度的一半。這些引物序列中的堿基可能與隨機(jī)序列區(qū)域或另一端的引物序列中的堿基配對，破壞序列的天然二級結(jié)構(gòu)，也降低了隨機(jī)庫序列的結(jié)構(gòu)多樣性格，即降低了核酸庫的容量。核酸庫容量的降低則可能導(dǎo)致篩選效率的降低。同時，較長的引物序列也可能與靶標(biāo)產(chǎn)生較強(qiáng)的非特異性格結(jié)合。而且，較長的引物序列也明顯增加核酸庫的合成成本，造成浪費(fèi)。

想了解更多的韓國SD產(chǎn)品及服務(wù)請掃描下方二維碼：我司還提供其它進(jìn)口或國產(chǎn)試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團(tuán)菌等試劑盒以及日本生研細(xì)菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產(chǎn)品。

二維碼掃一掃

【公司名稱】 廣州健侖生物科技有限公司
【】    楊永漢 

【】
【騰訊 】
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號二期2幢101-3室

【企業(yè)文化宣傳】

Mann and other small molecular weight ethanolamine (Mr = 61.08) as a target material, it is fixed to the magnetic beads. Added to the library of nucleic acid sequence and incubated for 30 min with shaking. The nucleic acid sequence not bound to ethanolamine was removed by the separation and enrichment effect of the magnetic lattice material. Subsequently, a buffer solution containing urea and EDTA was added to extract the nucleic acid sequence bound to the target material, Amplification enrichment, the final screening of 6 personality apatient body can be good. 5, Column chromatography Separation of the target substance in the resin chromatography column, the aptamer sequence library incubated with the target substance, the specific lattice combination of the sequence can stay on the column, to achieve the purpose of separation. Some people will be the target material fumonisin B1 immobilized on the gel resin affinity chromatography, into the library containing the nucleic acid sequence of the solution, the binding buffer repeatedly washed the column, the successful separation of fumonisin B1 dissociation constant 100 ± 30 nmol / L aptamer sequence. Among them, the first three methods are commonly used for the separation of macromolecular target substances, the latter two methods are commonly used for the separation of small molecule target substances.
Random nucleic acid library is usually obtained by chemical synthesis, but also by genomic DNA design and in vitro transcription and other access. Screening aptamers need to design a reasonable library of nucleic acids. Conventional random nucleic acid libraries are designed to contain primer sequences at both ends of the sequence, typically consisting of 20-60 bases in the middle. Increasing the length of the random sequence will result in exponential structural diversity of the nucleic acid molecules in the random library (4n, n is the number of bases in the random sequence). Reducing the primer sequence, avoiding the interference of the primer sequence on the secondary structure formation of the aptamer, reducing the non-specific lattice combination of the primer region and the target molecule, analyzing the sequence characteristics of the secondary library by high-throughput sequencing, changing the synthesis scheme of the nucleic acid library, The sequence or motif that binds to the target molecule can be increased.
First, the base sequence of the primer design At present, the conventional nucleic acid library used for screening has a total length of 60-100 nt, a primer sequence of 30-32 nt and a primer sequence of about half the length of the nucleic acid sequence. The bases in these primer sequences may be paired with bases in the primer sequence at the random sequence region or at the other end to disrupt the natural secondary structure of the sequence and to reduce the structural diversity of the randomized library sequence ie to reduce the capacity of the nucleic acid library . The bases in these primer sequences may be paired with bases in the primer sequence at the random sequence region or at the other end to disrupt the natural secondary structure of the sequence and to reduce the structural diversity of the randomized library sequence ie to reduce the capacity of the nucleic acid library . A reduction in the capacity of the nucleic acid pool may result in a reduction in screening efficiency. At the same time, longer primer sequences may also produce stronger non-specific lattice binding to the target. Moreover, longer primer sequences also significantly increase the synthesis cost of nucleic acid libraries, resulting in waste. Therefore, the nucleic acid library can be optimized by shortening the primer sequence or eliminating the primer sequence. In the double-RNAi screening method, the primer sequences of the designed nucleic acid libraries can fold and complement themselves, forming a double-stranded structure in the primer region, so that the non-specific lattice combination of the primer sequences and target molecules may be greatly reduced.